Table V.
Effect of aging and stress-induced senescence on thiol composition and synthesis in bean nodules
| Parameter | 3a | 5b | 7c | 3+Nd |
|---|---|---|---|---|
| Protein (mg g−1 fresh wt) | 17.2 ± 1.7 | 13.9 ± 0.2 | 6.1 ± 1.7 | 9.6 ± 1.3 |
| Shoot fresh wt (g plant−1) | 5.7 ± 0.4 | 22.7 ± 2.0 | 50.3 ± 4.5 | 5.4 ± 1.1 |
| Cys (nmol g−1 fresh wt) | 42.3 ± 1.8 | 28.7 ± 3.2 | 23.1 ± 2.8 | 17.2 ± 2.6 |
| γEC (nmol g−1 fresh wt) | 1.3 ± 0.8 | 0 | 0 | 0 |
| GSH (nmol g−1 fresh wt) | 180 ± 18 | 142 ± 17 | 126 ± 9 | 74 ± 10 |
| hGSH (nmol g−1 fresh wt) | 350 ± 29 | 204 ± 9 | 225 ± 12 | 39 ± 5 |
| γECS (nmol min−1 g−1 fresh wt) | 4.5 ± 1.1 | 0.7 ± 0.1 | 0.7 ± 0.4 | 1.9 ± 0.9 |
| GSHS (nmol min−1 g−1 fresh wt) | 0.9 ± 0.3 | 0 | 0 | 0.3 ± 0.1 |
| hGSHS (nmol min−1 g−1 fresh wt) | 10.6 ± 0.8 | 7.3 ± 1.4 | 2.0 ± 0.6 | 5.2 ± 0.3 |
Data are means ± se of three to six samples.
a
Nodule age, 3 weeks; plant age, 25 d; phenological stage, vegetative.
b
Nodule age, 5 weeks; plant age, 39 d; phenological stage, late flowering-early fruiting.
c
Nodule age, 7 weeks; plant age, 53 d; phenological stage, fruiting.
d
Nodule age, 3 weeks; plant age, 25 d (plus 4 d of treatment); treatment, 4 d with nitrate; phenological stage, early vegetative.