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. 2018 May 10;13(5):e0196797. doi: 10.1371/journal.pone.0196797

Fig 1. Phylogenetic tree of positive detected FHals from the Botany Bay metagenome in comparison to known FHals on amino acid level.

Fig 1

The tree was constructed using neighbour joining method, bootstrap 1000, with the alignment based on amino acids by MEGA7 [43] and MUSCLE [39]. 1–42: positive FHal hits from the Botany Bay metagenome; PyrH, PrnA, RebH, Thal: Tryptophan halogenases belonging to variant A and accept free substrate; PltA, CrpH, BhaA, CndH: FHals belonging to variant B and require carrier bound substrate. The amino acid sequences are shown in S3 Table.