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. 2018 May 10;13(5):e0197165. doi: 10.1371/journal.pone.0197165

Fig 5. Treatment of siRNA against GLE1, PLK1 or KIZ caused abnormal spindles.

Fig 5

Each value is the mean with SD of three independent experiments. Error bars represent the SD. p-values were calculated by one-way ANOVA followed by Dunnett’s test by comparison with the control. (*** = p<0.001, ** = p<0.01 and * = p<0.05). (A) Real-time PCR was performed using cDNA generated from cytoplasmic RNA of U2OS cells transfected with indicated siRNA. White bar: the detected value of real-time PCR. Gray bar: the detected value of microarray analysis. (B) Real-time PCR was performed to confirm the knock-down efficiency of GLE1, PLK1 and KIZ siRNAs. Cytoplasmic RNA of U2OS cells transfected with indicated siRNA was used to generate cDNA. Cells transfected with PLK1 siRNA were fixed after 24 h of culture, and GLE1 and KIZ were fixed after 48 h. (C-D) Staining of α-tubulin in cells treated with indicated siRNA. Cells were observed 24 h (C) or 48 h (D) after siRNA transfection. Chromosome was counterstained with DAPI. Scale bar, 20 μm.