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. 2018 May 10;13(5):e0197165. doi: 10.1371/journal.pone.0197165

Fig 7. The knock-down of DBP5 or IPPK resulted in reduced induction of IFNB1 caused by the poly(I:C) stimulation.

Fig 7

Each value is the mean with SD of three independent experiments. Error bars represent the SD. p-values were calculated by one-way ANOVA followed by Dunnett’s test by comparison with the control. (*** = p<0.001, ** = p<0.01 and * = p<0.05). (A) Expression levels of TLR3 and TRAF3 mRNAs from microarray data were validated by real-time PCR. Cytoplasmic total RNA was recovered from HeLa cells transfected with indicated siRNA. White bar: the detected value of real-time PCR. Gray bar: the detected value of microarray analysis. (B) Microarray data of IFNB1 mRNA level suggest that the knock-down of DBP5, GLE1 and IPPK does not impair the cytoplasmic mRNA expression of IFNB1 at the basal expression level. (C) Real-time PCR was performed using cDNA generated from whole-cell RNA extracted by poly(I:C)-treated HeLa cells. Each siRNA transfection significantly reduces mRNA of an indicated gene. (D) IFNB1 expression level detected by real-time PCR using HeLa cells. HeLa cells transfected by the indicated siRNA were cultured for 45 h, then treated with poly(I:C). Cells were fixed 3 h after poly(I:C) treatment, and RNA was isolated from whole-cell lysate.