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Podocytes were pretreated with different concentrations (10−7,10−6 or 10-5mol/L) of Butaprost (n = 4 each dose) for 30 min and were treated with 5 ng/ml TGF-β1 for 24 h. Nephrin, podocin and CD2AP were determined at the mRNA and protein level.(A:RT-qPCR, B: Western blot). aP<0.05 compared to the control group, bP<0.05 compared to the TGF-β1 group.
