Fig 8. The effect of Butaprost on PI3K, Akt, and caspase3 expression in TGF-β1- induced podocytes.

Podocytes were pretreated with different concentrations (10⁻⁷,10⁻⁶, or 10^-5mol/L) of Butaprost (n = 4 each dose) for 30 min, then treated with 5 ng/ml TGF-β1 for 24 h. p-PI3K, p-Akt, and cleaved-caspase 3 were determined at the protein level.(A: p-PI3K, B: p-Akt, C: cleaved-caspase3) ^aP<0.05 compared to the control group, ^bP<0.05 compared to the TGF-β1 group.