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. 2018 Apr 30;14(4):e1007039. doi: 10.1371/journal.ppat.1007039

Fig 6. CD40 stimulation rescues EBNA3A and EBNA3C deficient EBV infected cells in vitro.

Fig 6

Purified human B cells were infected with wt, 3AKO or 3CKO and cultured with irradiated fibroblasts either expressing CD40L or not (n = 6). (A, B) The change in the frequency of living infected cells (GFP+) cells between the culture condition with CD40L or without CD40L for wt, 3AKO or 3CKO infected B cells (A) 2 and (B) 3 weeks p.i., determined with 7AAD life-dead stain by flow cytometry. (C, D) The change in the frequency of early apoptotic Annexin V+ 7AAD- cells between the culture condition with CD40L or without CD40L for wt, 3AKO or 3CKO infected B cells (C) 2 and (D) 3 weeks p.i., determined by flow cytometry. (A-D) Pooled data from 3 experiments represented with the mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001, paired t-test.