Fig 1. Q223L point mutation resulted in a constitutively active CT2 (CT2^CA).

(A) BODIPY-GTP assay for detecting the GTP-binding and GTPase activity of His-CT2 and His-CT2^CA proteins. Data are shown as means of four replicates and error bars represent S. D. (B) CT2^CA did not interact with a Gβγ dimer in a Y3H assay. Yeast growth on synthetic complete -Trp -Leu (-LW) medium confirmed transformation and cell viability. Interactions were assayed on SC -Trp -Leu -His (-LWH) medium supplemented with 1 mM 3-AT.