Figure 4.
Changes in the apparent Ki for l-malate, PEPc kinase activity, and translatable kinase mRNA at the start of the photoperiod after a night in ambient or anaerobic conditions. Leaves that had been maintained in an atmosphere of N2 overnight to prevent malate accumulation were transferred to ambient air at the start of the photoperiod (○). Control leaves were maintained in ambient air (●). Samples for PEPc and PEPc kinase assays and RNA isolation were taken simultaneously from the same leaves at intervals over the light period. Kinase activity and translatable mRNA values are expressed as percentages of the maximum reached during the photoperiod. The results are from duplicate experiments.