REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-PGK1 polyclonal	Antibodies-online.com	Cat# ABIN568371
Rabbit anti-IAPP polyclonal	Sigma Aldrich	Cat# HPA053194
Donkey anti-mouse secondary 800CW dye conjugate	LI-COR	Cat# 926-32212
Donkey anti-rabbit secondary 680CW dye conjugate	LI-COR	Cat# 926-68073
Rabbit anti-mouse secondary polyclonal HRP conjugate	Sigma Aldrich	Cat# A-9044
Bacterial and Virus Strains
Escherichia coli DH5alpha	ATCC	Cat# 69021
Biological Samples
Chemicals, Peptides, and Recombinant Proteins
Nourseothricin dihydrogen sulfate (ClonNAT)	Werner BioAgents	Cat# 5.005.000
G418 (geneticin)	Life Technologies	Cat# 10131027
B-Estradiol	Sigma Aldrich	Cat# E8875-5G
Propidium iodide	Sigma Aldrich	Cat# P4864
Lopinavir	Sigma Aldrich	Cat# SML1222-10MG
Yeast nitrogen base	VWR	Cat# 90004-146
Complete supplement mixture (CSM) media	Sunrise Science Products	Cat# 1001-100
CSM lacking uracil	Sunrise Science Products	Cat# 1004-100
CSM lacking leucine	Sunrise Science Products	Cat# 1005-100
CSM lacking histidine	Sunrise Science Products	Cat# 1006-100
CSM lacking uracil and histidine	Sunrise Science Products	Cat# 1009-100
Critical Commercial Assays
TruSeq RNA Library Prep Kit	Ilumina
Deposited Data
RNASeq	GEO	GSE104172
Experimental Models: Cell Lines
INS-1 823/13	Newgard Lab	CVCL_7226
Experimental Models: Organisms/Strains
S. cerevisiae: Strain background: W303	ATCC	Cat# 201238
S. cerevisiae: Strain background: BY4741	ATCC	Cat# 201388
Oligonucleotides
Please see Table S5
Recombinant DNA
Please see Table S6 for all plasmids
Software and Algorithms
GraphPad Prism	GraphPad Software	https://www.graphpad.com/scientific-software/prism/
SGA Score algorithm	Baryshnikova et al, 2010
Cell Profiler	Kamentsky et al., 2011	cellprofiler.org
TopHat	Trapnell et al., 2009	https://ccb.jhu.edu/software/tophat/index.shtml
htseq-count	Anders et al., 2015
EdgeR	Robinson et al., 2010
Picard	Broad Institute	http://broadinstitute.github.io/picard/
Burrows-Wheeler Aligner (BWA)		http://bio-bwa.sourceforge.net/
GATK	Broad Institute	https://software.broadinstitute.org/gatk/
Variant Effect Predictor (VEP)	McLaren et al., 2016	https://useast.ensembl.org/info/docs/tools/vep/index.html
EPACTS		http://csg.sph.umich.edu/kang/epacts/download/index.html
EIGENSTRAT		https://github.com/DReichLab/EIG/tree/master/EIGENSTRAT
MATLAB	The Mathworks Inc.	https://www.mathworks.com/products/matlab.html
FlowJo v8	FlowJo, LLC.	https://www.flowjo.com/solutions/flowjo/downloads
ImageJ		https://imagej.net/Welcome
Other