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. Author manuscript; available in PMC: 2018 May 10.
Published in final edited form as: Biochemistry. 2016 Aug 17;55(34):4787–4797. doi: 10.1021/acs.biochem.6b00430

Figure 2.

Figure 2

Predicted secondary structure of CRAC336WT in solution or in membranes composed of either 100% POPC or 60% POPC and 40% Chol, determined with DICHROWEB using CONTIN/LL and the SP175 or SMP180 reference set. The bar graph is split into an α-helical structure section (left) and a β-sheet structure section (right). Each bar graph represents data averaged over three independent experiments. The level of significance was determined using an unpaired two-sample t test. ***P ≤ 0.001; **P ≤ 0.01; *P ≤ 0.05; NS, P > 0.05.