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. Author manuscript; available in PMC: 2018 May 10.
Published in final edited form as: Biochemistry. 2016 Aug 17;55(34):4787–4797. doi: 10.1021/acs.biochem.6b00430

Table 2.

Thermodynamic Parameters of Interactions of LtxA, CRAC336WT, and CRAC336SCR with Chola

KD (M) ΔH (kJ/mol) ΔS (kJ mol−1 K−1) −TΔS (kJ/mol) ΔG (kJ/mol)
LtxA and POPC 8.75 × 10−4 15.24 0.109 −32.99 −17.8
LtxA and Chol 2.31 × 10−10 −29.76 0.086 −26.15 −55.9
CRAC336WT and POPC 3.81 × 10−3 32.58 0.155 −46.8 −14.2
CRAC336WT and Chol 5.05 × 10−8 2.81 0.152 −46.2 −43.4
CRAC336SCR and POPC 5.36 × 10−5 −7.65 0.057 −17.27 −24.9
CRAC336SCR and Chol 4.07 × 10−4 −5.45 0.047 −14.24 −19.7
a

The affinity and thermodynamic properties were obtained using the independent or two-site model, as described in Materials and Methods.