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. Author manuscript; available in PMC: 2018 Jun 20.
Published in final edited form as: Sci Transl Med. 2017 Dec 20;9(421):eaai7635. doi: 10.1126/scitranslmed.aai7635

Fig. 5. Differential expression of CD33 isoforms in MDM.

Fig. 5

(A) In our model system, we found a significant effect of genotype on CD33 expression: an increase in CD33M mRNA expression (***P = 0.0009) and decreased CD33m mRNA expression (****P < 0.0001) in MDMi in a dose dependent fashion relative to the rs3865444C risk allele in the fluidigm dataset. One-way ANOVA with Tukey’s post hoc test, N = 95. (B) Western blot analysis shows a significant effect of genotype on CD33M protein expression in monocytes (*P = 0.034) and MDMi (**P = 0.009) from a second set of individuals (N = 16). However, a significant effect of genotype on CD33m protein expression was only observed in MDMi (*P = 0.016) and not monocytes (P = 0.124). A representative western blot displays the data from four subjects, with each subject being in a column. The genotype of the subject is included at the top of the column. (C) The genotype-dependent difference in CD33 surface expression (P = 0.001) and (D) FITC-dextran uptake (P = 0.047) was further confirmed in MDMi using high content imaging. Each dot represents a biological replicate. Student’s t-test performed for B–D.