Skip to main content
. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Hypertension. 2018 Apr 23;71(6):1248–1257. doi: 10.1161/HYPERTENSIONAHA.118.11127

Figure 1.

Figure 1

Genotype confirmation using polymerase chain reaction (PCR) from tail snip samples with analysis indicating presence or absence of Cre-recombinase and SOCS3flox/flox and immunofluorescence analysis. (A) Top: +/+ indicates a homozygous, +/− heterozygous results and WT, wild-type based on the 3 control bands for WT, +, and – primers (far right). Bottom: + expresses Cre-recombinase and – does not express Cre-recombinase based on + and − control bands (far right). Positive (+) and negative (−) DNA samples. (B) SOCS3 mRNA levels quantified by qRT-PCR in hypothalamic and cortex fractions of SOCS3-Nestin-Cre and SOCS3flox/flox control mice (n=4/per group). (C) 20x view of representative immunohistochemical staining of p-STAT3 expression. (D) Quantification of pSTAT3 positive cells in arcuate nucleus of homozygous SOCS3-Nestin-Cre and SOCS3flox/flox control mice (n=3 per group). *p<0.05 compared to SOCS3flox/flox mice.