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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Hypertension. 2018 Apr 9;71(6):1133–1142. doi: 10.1161/HYPERTENSIONAHA.118.10952

Figure 3.

Figure 3

Effect of Ang-II on renal fibrosis and α-smooth muscle actin (α-SMA) protein expression in the kidney of Tβ4 KO mice. (A) Representative images of renal interstitial fibrosis. Red color indicates total collagen content by picrosirius red staining (PSR). Images were captured using a 20× microscope objective. Scale bar = 100 μM. In Ang-II infusion, total renal collagen content increased significantly in Tβ4 KO mice, compared with that in the WT. (B) Quantitative data for Figure 3A. (C) Total renal collagen content was further quantified by a different method utilizing a biochemical hydroxyproline assay. (D) Top figure: At the basal level, no significant difference was observed in renal α-SMA expression; Ang-II infusion significantly increased α-SMA expression in Tβ4 KO mice, compared with that in the WT. Bottom figure shows the quantitative data. All data are expressed as the mean ± SEM, n = 6-10 in each group.