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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: Mol Oral Microbiol. 2018 Feb 20;33(3):212–223. doi: 10.1111/omi.12214

Figure 4. Effect of 5-FU treatment on neutrophil transmucosal migration.

Figure 4

A: Immunofluorescence staining of MPO+ differentiated HL-60 cells (red) in untreated tissues (CTR) or tissues treated with 5-FU (10 µM) for 16h. Cell nuclei were counterstained with the nucleic acid stain Hoechst 33258 (blue). A representative immunofluorescence image is shown, from each condition tested in triplicate, in one of two independent experiments. Bars=20µm. B: Quantitative assessment of HL-60 cell transmigration through epithelial cell layers in untreated (5-FU(−)) versus 5-FU-treated tissues ((5-FU(+)) shown above. Bars corresponding to the left Y-axis represent MPO activity in tissue lysates as assessed by a colorimetric assay. Error bars represent one standard deviation of the mean of triplicate tissues in two independent experiments. MPO+ cell numbers, as assessed by immunofluorescence in 6 microscopic fields per condition, are plotted against the Y-axis to the right, with error bars indicating one SEM. *p<0.001