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. Author manuscript; available in PMC: 2019 Jun 1.

Published in final edited form as: Mol Oral Microbiol. 2018 Feb 20;33(3):212–223. doi: 10.1111/omi.12214

A: C. albicans SN425, C. glabrata GDH2269, S. oralis 34 and S. mitis 49456 were inoculated on mucosal constructs that had been pre-treated with 10µM 5-FU for 16h. Biofilms were allowed to grow for 16h after treatment. Untreated mucosal constructs served as control. Biofilm surface area was quantified microscopically using Image J software, in 4–8 microscopic fields per biofilm, tested in triplicate, in two independent experiments. Results are expressed as mean fold biofilm surface area in 5-FU-treated over untreated tissues. *p<0.005. B: C. albicans biofilms growing on untreated (CTR) or 5-FU-treated mucosal constructs for 6h or 16h. Immunofluorescence images are showing C. albicans biofilms (green), epithelial cell E-cadherin (red) and nuclei (blue). Arrows indicate tissue invasion by fungal organisms. C: Immunofluorescence images are showing C. glabrata (green), S. oralis (red) and S. mitis (red) biofilms growing on untreated (CTR) or 5-FU-treated mucosal constructs for 16h. Cell nuclei are shown in blue. Arrow points to an intraepithelial cluster of C. glabrata. A representative immunofluorescence image is shown, from each condition tested in triplicate, in one of two independent experiments. Bars=50µm.

A: C. albicans SN425, C. glabrata GDH2269, S. oralis 34 and S. mitis 49456 were inoculated on mucosal constructs that had been pre-treated with 10µM 5-FU for 16h. Biofilms were allowed to grow for 16h after treatment. Untreated mucosal constructs served as control. Biofilm surface area was quantified microscopically using Image J software, in 4–8 microscopic fields per biofilm, tested in triplicate, in two independent experiments. Results are expressed as mean fold biofilm surface area in 5-FU-treated over untreated tissues. *p<0.005. B: C. albicans biofilms growing on untreated (CTR) or 5-FU-treated mucosal constructs for 6h or 16h. Immunofluorescence images are showing C. albicans biofilms (green), epithelial cell E-cadherin (red) and nuclei (blue). Arrows indicate tissue invasion by fungal organisms. C: Immunofluorescence images are showing C. glabrata (green), S. oralis (red) and S. mitis (red) biofilms growing on untreated (CTR) or 5-FU-treated mucosal constructs for 16h. Cell nuclei are shown in blue. Arrow points to an intraepithelial cluster of C. glabrata. A representative immunofluorescence image is shown, from each condition tested in triplicate, in one of two independent experiments. Bars=50µm.