Figure 2.

SDS-PAGE and immunoblot analysis of control and mutant ACC synthase. A, SDS-PAGE of protein samples from different stages of purification. Lane 1, Soluble cell extract; lane 2, protein sample eluted from DEAE expanded bed; lane 3, protein sample eluted from the hydroxylapatite column. B, The immunoblot was color-developed with the primary antibody raised against tomato del-1 ACC synthase, followed by the secondary antibody conjugated with alkaline phosphatase. ACC synthase is marked by arrows.