Figure 3. miR-215 is increased in ulcerative colitis-associated colon cancer.

(A) Expression of miR-215 was evaluated by real time PCR in sporadic colon cancer, tissue adjacent to sporadic colon cancer, UC-associated colon cancer, non-dysplastic tissue adjacent to UC cancers (n = 9/group). (B) miR-215 expression in isolated colonocyte and stromal fractions from normal controls, UC-associated neoplasia, and non-dysplastic UC tissue adjacent to a neoplastic lesion. Fold change is compared to expression of miR-215 in whole tissue normal control biopsies. Error bars demonstrate standard error of the mean. (C) In situ hybridization using U6 or miR-215 in normal controls or UC-associated colon cancers as indicated. Images are taken at 10× magnification with insets representing 40× magnification.