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Western-blot analysis of peroxisomes from senescent pea leaves. Samples were subjected to SDS-PAGE and then transferred to polyvinylidene difluoride membranes and incubated with a polyclonal antibody against pea NADP-ICDH (1/4,000 dilution). Lane a, Crude extract of pea leaves (50 μg of protein); lane b, matrices of pea leaf peroxisomes (45 μg of protein). Molecular mass standards are indicated on the left in kD.
