Table II.
Immunocytochemical labeling intensity of NADP-ICDH in young (15 d) and senescent (50 d) pea leaf cells
Cellular Compartment | Young Leaves | Senescent Leaves |
---|---|---|
no. of gold particles μm−2 | ||
Chloroplast | 4.5 ± 0.3 | 12.6 ± 0.6 (B) |
Mitochondrion | 10.0 ± 0.5 | 24.0 ± 1.8 (B) |
Peroxisome | 11.0 ± 0.7 | 22.2 ± 3.0 (A) |
Cytoplasm | 1.1 ± 0.1 | 2.0 ± 0.2 (A) |
Cell wall | 0.8 ± 0.2 | 0.9 ± 0.2 (NS) |
NADP-ICDH labeling density is given as the number of gold particles per square micrometer. Two separately embedded blocks were used to cut sections of each class of leaves, which were photographed from random fields. An average of 17 photographs were used for quantitative analysis for each type of leaves, and results are given as the means ± se. Differences from young leaf values were significant at: P ≤ 0.01 (A); P ≤ 0.001 (B). NS, Not significant.