Table 4. Detailed aspects for each antibody used in immunohistochemistry.
Protein | Antigen retrieval | Antibody | Antibody dilution and incubation time |
---|---|---|---|
MCT1 | Citrate buffer (0.01 M, pH = 6), 98° C, 20′ | AB3538P Chemicon International |
1:1000, overnight |
MCT2 | Citrate buffer (0.01 M, pH = 6), 98° C, 20 | sc-50322 Santa Cruz Biotechnology |
1:200, 2 hours |
MCT4 | Citrate buffer (0.01 M, pH = 6), 98° C, 20′ | sc-50329 Santa Cruz Biotechnology |
1:500, 2 hours |
CD147 | EDTA (1 mM, pH = 8), 98° C, 20’ | sc-71038 Santa Cruz Biotechnology |
1:400, overnight |