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. 2018 Apr 17;9(29):20323–20338. doi: 10.18632/oncotarget.24859

Figure 4.

Figure 4

(A) RNA-Seq was performed on MYCN3 cells under different p53 and MYCN high conditions. Treatment conditions were compared to determine the effect of low and high MYCN levels on p53 response. p53 activated and repressed genes under high and low MYCN conditions were determined and shown with Venn diagrams. (B) Waterfall plots of the relative changes in gene expression of common genes shows the p53-regulated genes under low MYCN vs. high MYCN conditions. High MYCN levels relatively repressed the p53-up-regulated genes and relatively de-repressed the p53-down-regulated genes. (C, D) Functional annotation analysis of differentially expressed genes using the DAVID Bioinformatics platform. Representative genes analyzed are shown in boxes. (E) Gene Set Enrichment Analysis (GSEA) shows a statistically significant and robust interaction between the MYCN and p53 transcriptional programs. Genes induced by MYCN in either low or high p53 conditions are suppressed in the p53 transcriptional response regardless of MYCN levels (Normalized Enrichment Score [NES]<0, Q<0.25). (F) Genes repressed by p53 in either low or high MYCN conditions are induced in the MYCN transcriptional response regardless of p53 levels (NES>0, Q<0.25).