Table 3. miRNAs analysed in the replica/validation phase by qPCR and stratified for BC invasiveness and grade.
| Replica | Replica/Validation | ||||||
|---|---|---|---|---|---|---|---|
| miRNA | Log2 Fold Change | P | adj P | Log2 Fold Change | P | adj P | |
| MIBC | amiR-21-5p | 0.65 | 0.309 | 0.386 | 0.73 | 0.271 | 0.338 |
| amiR-106b-3p | 1.33 | 0.069 | 0.116 | 1.58 | 0.054 | 0.107 | |
| bmiR-30a-5p | –2.43 | 0.002 | 0.011 | –2.12 | 0.006 | 0.017 | |
| blet-7c-5p | –1.29 | 0.144 | 0.205 | –1.04 | 0.234 | 0.312 | |
| bmiR-486-5p | 2.55 | 0.026 | 0.058 | 2.75 | 0.017 | 0.038 | |
| cmiR-205-5p | 1.84 | 0.005 | 0.017 | 1.92 | 0.012 | 0.029 | |
| miR-451a | 3.13 | 0.011 | 0.031 | 3.57 | 0.004 | 0.014 | |
| miR-25-3p | 1.97 | 0.005 | 0.017 | 2.21 | 0.004 | 0.014 | |
| miR-7-1-5p | 2.49 | 0.002 | 0.011 | 2.74 | 0.001 | 0.012 | |
| miR-146a-5p | 1.00 | 0.109 | 0.168 | 1.14 | 0.131 | 0.193 | |
| NMIBC G1 + G2 |
dmiR-30c-2-5p | –1.08 | 0.022 | 0.144 | –0.58 | 0.149 | 0.248 |
| dmiR-151a-3p | –0.26 | 0.494 | 0.657 | 0.37 | 0.265 | 0.353 | |
| dmiR-30a-5p | –0.52 | 0.304 | 0.656 | –0.28 | 0.482 | 0.508 | |
| blet-7c-5p | –0.18 | 0.764 | 0.858 | 0.25 | 0.553 | 0.553 | |
| bmiR-486-5p | 1.67 | 0.059 | 0.197 | 1.63 | 0.017 | 0.073 | |
| cmiR-205-5p | 1.76 | 0.000 | 0.007 | 1.60 | 0.000 | 0.002 | |
| let-7i-5p | 0.31 | 0.427 | 0.656 | 0.76 | 0.026 | 0.076 | |
| NMIBC G3 | amiR-21-5p | 1.36 | 0.005 | 0.008 | 1.29 | 0.007 | 0.011 |
| amiR-106b-3p | 1.67 | 0.001 | 0.002 | 1.94 | 0.000 | 0.001 | |
| bmiR-30a-5p | –0.97 | 0.127 | 0.149 | –0.78 | 0.178 | 0.210 | |
| blet-7c-5p | 1.25 | 0.088 | 0.110 | 1.13 | 0.097 | 0.121 | |
| bmiR-486-5p | 3.13 | 0.001 | 0.002 | 3.37 | 0.000 | 0.001 | |
| dmiR-30c-2-5p | –1.56 | 0.001 | 0.002 | –1.19 | 0.019 | 0.027 | |
| dmiR-151a-3p | 1.22 | 0.001 | 0.002 | 1.41 | 0.001 | 0.002 | |
| miR-200c-3p | 1.53 | 0.000 | 0.001 | 1.63 | 0.000 | 0.001 | |
| miR-4448 | na | na | |||||
| miR-183-5p | 1.96 | 0.000 | 0.000 | 1.98 | 0.000 | 0.000 | |
| miR-185-5p | 0.86 | 0.015 | 0.021 | 0.87 | 0.022 | 0.029 | |
| miR-98-5p | 0.34 | 0.473 | 0.526 | 0.00 | 0.995 | 0.995 | |
| miR-148b-3p | 0.09 | 0.887 | 0.887 | –0.03 | 0.951 | 0.995 | |
| miR-10b-5p | –1.69 | 0.018 | 0.024 | –1.64 | 0.005 | 0.008 | |
| miR-224-5p | 2.76 | 0.000 | 0.000 | 2.97 | 0.000 | 0.000 | |
Significant results in bold. Abbreviations: FDR false discovery rate, PP predictive power, MIBC muscle-invasive bladder cancer, NMIBC non-muscle invasive bladder cancer.
amiRNAs in common between NMIBC G3 and MIBC.
bmiRNAs in common among NMIBC G1 + G2, NMIBC G3, and MIBC.
cmiRNAs in common between NMIBC G1 + G2 and MIBC.
dmiRNAs in common between NMIBC G1 + G2 and NMIBC G3.