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. 2018 Apr 17;9(29):20640–20657. doi: 10.18632/oncotarget.25045

Figure 3. CD44 outside-in signaling controls invadopodia formation.

Figure 3

(A) Cell lysates of GSCs transfected with NT- or CD44- siRNA were analyzed by Western blotting and probed for CD44 and Actin. SiRNA silencing strategy was efficient leading to 75% decrease in CD44 protein levels; ***P < 0.01 (n = 3). (B) CD44-siRNA knockdown affected GSCs spreading capacity on Matrigel-coated substrates. (C) NT- or CD44- siRNA transfected GSC-GFP cells were fixed and stained with rhodamine phalloidin and DAPI. CD44 rapid depletion strongly affected cells ability to form invadopodia. CD44 knockdown significantly reduced the % of GSCs with invadopodia; *P < 0.05 (n = 3). Graphs presented as means ± SD. Differences with a probability level P < 0.05 were considered significant in one-way ANOVA. Bars: (B) 50 μm; (C) 30 μm.