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. 2018 Apr 17;9(29):20640–20657. doi: 10.18632/oncotarget.25045

Figure 5. GSCs alone or in cooperation with ECs can contribute to GBM vascularization.

Figure 5

(A) GSCs formed vascular networks reminiscent of normal endothelium over the gelled matrix. (B) Representative images extracted from a time series of GSC-GFP cells forming tubes over the gelled matrix. (C) GSC-derived tubes were fixed and stained with a CD31 antibody (red). GSC cells did not express the endothelial marker CD31. (D) Representative confocal images of GFP expressing GSCs plated on damaged HUVEC-derived tubes and stained for DAPI (blue) and rhodamine phalloidin (red). GSC-GFP cells efficiently restored the destroyed tubes around the HUVEC-remaining branch points. (E) A mixed population of GSC-GFP and HUVEC cells was seeded on a gelled substrate in serum-free neurobasal medium. To discriminate GSCs (green) from HUVECs cells, the tubes were fixed and stained with a CD31 antibody (red). GSCs physically interacted with HUVECs and formed intact vascular tubes. Bars: (A) 100 μm; (B) 50 μm; (C) 50 μm; (D) 50 μm; E) 100 μm.