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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Nat Protoc. 2017 Nov 9;12(12):2478–2492. doi: 10.1038/nprot.2017.124
Step Problem Possible Reason Solution
7 There are no present calls (1 values) in the binarized data Peak calls were provided opposed to aligned reads and the ‘-peaks’ flag was not specified Use aligned reads or add the ‘-peaks’ option
10 Error message that the Information initialization strategy can only support a certain number of states The binarization might not have been done correctly and there are no present calls. Alternatively more states are being asked for than there are observed combination of marks If there are no present calls see troubleshooting for step 7. If there are more states than combination of marks either reduce the number of states or instead use the Random initialization strategy
10 Error message indicating insufficient memory Java does not have access to enough memory or there is insufficient memory available on the system Increase the memory Java has access to by increasing the value associated with the ‘-mx’ flag. Alternatively if using the ‘-p’ flag set it to a small non-zero integer value to reduce the number of processors and thus memory used simultaneously at a cost of additional runtime.