Fig. 4.

Stable overexpression of full-length TSP2, but not of the short TSP2 variant, in A431 cells inhibits intradermal tumor growth and angiogenesis. a Tumor growth was measured in mice with A431 tumors expressing either TSP2 or sTSP2 (n = 12 per group). b Representative pictures of NOD-SCID mouse skin flaps containing intradermally injected A431 tumors transfected with control vector, full-length TSP2 (TSP2), or the short TSP2 variant (sTSP2). Arrows indicate tumor borders. Scale bars = 5 mm. c Tumor sections were stained with MECA-32 (vascular marker; green) and with Hoechst (nuclear marker; blue). d Quantification of tumor area covered by blood vessels for tumors of similar size (n = 12 for control and sTSP2, n = 10 for TSP2; 200–600 mm³). Data are presented as mean ± SD. All comparisons for testing for significance were compared to A431-control. *p < 0.05, ***p < 0.001. Scale bars = 100 μm