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. 2018 May 10;9(5):552. doi: 10.1038/s41419-018-0551-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — PC3 and H1299 cells were transfected with either siControl or one of two individual siRNAs targeting AGO2 (siAGO2#1 and siAGO2#2) for 48 h. a AGO2, PTBP1, MCL1, and β-actin levels were detected by western blotting. Relative MCL1 band intensity was quantified and normalized against β-actin. b Relative MCL1, PTBP1, and AGO2 mRNA levels were quantified by RT-qPCR using ACTB as the internal control. Data is shown as mean ± SEM, n = 3. The statistical significance was determined using unpaired Student’s t-test where *p < 0.05; **p < 0.01; ***p < 0.001.