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. 2018 May 10;8:7493. doi: 10.1038/s41598-018-25817-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification of peptides in infarcted and healthy areas by ROI analysis. The box plot shows differences and the distribution of intensity of all pixels in a m/z image between infarcted and healthy area. Box plot: upper horizontal line of box, 75^th percentile; lower horizontal line of box, 25^th percentile; horizontal bar within box, median; upper horizontal bar outside box, 90^th percentile; lower horizontal bar outside box, 10^th percentile. The histogram shows differences and the frequency of intensity of all pixels of the m/z image between infarcted and healthy areas. The histogram vertical axes represent the frequency of pixels. The horizontal axes represent intensity. (a) Sample 1, (b) sample 2. The two photos on the left represent individual ROIs. The red lines demarcate the border of the ROI for the endocardial infarcted lesion (D) in the left side of the tissues and of the residual normal area (N) in the right side of intact area. The yellow lines demarcate the ROI of the healthy lesion.