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. 2018 May 10;8:7470. doi: 10.1038/s41598-018-25869-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MS/MS spectrum of the m/z 1134 PAGPE anion and 2D-TLC chromatogram. (A) Dissociation and MS/MS spectrum of the m/z 1134 deprotonated anion. (B) The 2-D-TLC sheet was stained with 0.02% primuline. Representative fluorescent spots were analyzed by MS. The types of lipids recovered from silica gels are marked on the chromatogram. Positive MS spectrum of lipids recovered at the PAGPE spot is shown.