Fig. 3. cZRANB1 knockdown directly regulates Müller cell but indirectly regulates RGC function in vitro.

a Müller cells were transfected with scramble (Scr) shRNA, cZRANB1 shRNA1, cZRANB1 shRNA2, or left untreated (Ctrl) for 24 h. qRT-PCR assays were conducted to detect cZRANB1 expression (n = 4, *P < 0.05 vs. Ctrl). b The viability of Müller cells was detected by MTT method. The data were expressed as the relative change compared with Ctrl group (n = 4, *P < 0.05 vs. Ctrl). c Ki67 staining was used to detect Müller cell proliferation (n = 4, *P < 0.05 vs. Ctrl group). Scale bar: 20 μm. d RGCs were co-cultured with wild-type Müller cells (Ctrl), Scr shRNA-transfected Müller cells, cZRANB1 shRNA1-transfected Müller cells, or cZRANB1 shRNA2-transfected Müller cells, and then treated with H₂O₂ (100 μm) for 24 h. PI staining and quantitative analysis was performed to detect apoptotic RGCs (n = 4, *P < 0.05 vs. Ctrl)