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. 2018 May 10;9(5):540. doi: 10.1038/s41419-018-0597-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a The expression of nuclear control transcript (U6), cytoplasmic control transcript (GAPDH), and cZRANB1 was detected by qRT-PCRs in the nuclear and cytoplasmic fraction of Müller cell (n = 4). b The full sequence of cZRANB1 was cloned into the pGL3 Luciferase Reporter to construct LUC-cZRANB1 vector. Müller cells were co-transfected LUC-cZRANB1 with different miRNA mimics. Luciferase activity was detected by the dual luciferase assay at 24 h after transfection (n = 4, *P < 0.05 vs. Ctrl group). c The 3′-end biotinylated miRNA duplexes were transfected into Müller cells. After the streptavidin capture, the amount of cZRANB1 and cZNF532 (negative control) in the input and bound fractions were detected by qRT-PCRs (n = 4). d The 3′-end biotinylated cZRANB1 or cZNF532 (negative control) was transfected into Müller cells. After the streptavidin capture, the amount of miR-217 and miR-335 (negative control) in the input and bound fractions were detected by qRT-PCRs (n = 4)