Fig. 6. cZRANB1-miR-217-RUNX2 is involved in regulating Müller cell function.

a, b Müller cells were transfected with scramble (Scr) miRNA mimic, miR-217 mimic, or left untreated for 24 h. MTT assays a and Ki67 staining b was conducted to detect cell viability and proliferation (n = 4, *P < 0.05 vs. Ctrl group). Scale bar: 20 μm. c, d Two days prior to the co-culture, Müller cells were transfected with miR-217 mimic, Scr mimic, or left untreated. At the day of co-culture, Müller cells were cultured on the upper chamber of Transwell. RGCs were cultured on the below chamber. RGCs was cultured alone as the control group (Ctrl). They were then incubated with H₂O₂ (c, 100 μm) or glutamate (d, 3 mM) for 24 h. After the above-mentioned treatments, the upper chamber was removed. PI staining and quantitative analysis was used to detect apoptotic RGCs (n = 4, *P < 0.05 vs. Ctrl group; ^#P < 0.05 Müller cell + Scr miRNA mimic vs. Müller cell + miR-217 mimic group). All data were from at least three independent experiments