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. 2018 May 10;8:7449. doi: 10.1038/s41598-018-25929-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of XSECC on neural injury in BCCAO rats. Four non-overlapping images (400× magnification) within hippocampal CA1 of each animal were taken for the analysis of HE staining and NeuN immunofluorescence respectively. (a) Representative HE staining of the unilateral hippocampus (Scale bar: 200 μm) and hippocampal CA1 area (Scale bar: 50 μm) from sham, model and XSECC group rats. Red rectangle represented the region of interest located in the CA1 area of hippocampus. The neurons are shrinkaged and dark-stained in the model group rats (red arrows). (b) Immunofluorescence staining of NeuN in the hippocampus CA1 area (Scale bar: 50 μm). (c,d) Analysis of pyramidal cells and NeuN expression in the hippocampal CA1 area respectively. ^##P < 0.01 vs. sham group; **P < 0.01 vs. model group (one-way ANOVA followed by LSD’s post hoc test, n = 6 per group).