Table 1.
“Motility” proteins displaying differential spectral-counts between normo-gravity and microgravity conditions
| Molecular function enrichment item (FunRich) | Uniprot protein ID | Protein name | EmPAI quantification | P-value | Trend | ||
|---|---|---|---|---|---|---|---|
| mean_ctrl | mean_Sμg | ||||||
| (i) Adhesion proteins | TLN 1 | Talin | 0,188123068 | 0.070200944 | 2,11E-06 | DOWN (***) | |
| ITGB 1 | Integrin beta 1 | 0,06516658 | 0,044034984 | 0,0507549 | DOWN | ||
| ITGA3 | Integrin alpha-3 | 0,006907204 | 0,004317346 | 0,0155 | DOWN (*) | ||
| ZYX | Zyxin | 0,131873677 | 0,068814834 | 0,039799 | DOWN (*) | ||
| (ii) Protrusion dynamic actin network | Arp2/3 complex | ARPC 5 | Actin-related protein 2/3 complex subunit 5 (p16) | 0,074171194 | 0,162733776 | 0,046479 | UP (*) |
| ARPC 3 | Actin-related protein 2/3 complex subunit 3 (p21) | – | 0120347326 | – | UM | ||
| ARPC 2 | Actin-related protein 2/3 complex subunit 2 (p34) | 0,167532621 | 0,065708433 | 0,000261 | DOWN (***) | ||
| ACTR 3 | Actin-related protein 3 (ARP3) | 0,049531658 | 0,033574567 | 0,012523 | DOWN (*) | ||
| ACTR 2 | Actin-related protein 2 (ARP2) | 0,051803205 | – | – | UN | ||
| ARPC 4 | Actin-related protein 2/3 complex subunit 4 (p20) | 0,040176784 | 0,172100443 | 6,68E-08 | UP (***) | ||
| ARPC 1B | Actin-related protein 2/3 complex subunit 1B (p41 ARC) | 0,022004409 | – | – | UN | ||
| Cytoskeletal organization proteins | ENAH | Protein enabled homolog | 0,011537141 | 0,007866219 | 0,022371 | DOWN (*) | |
| VASP | Vasodilator-stimulated phosphoprotein | – | 0,014752477 | – | UM | ||
| CFL 1 | Cofilin-1 | 0,742005965 | 0,304803386 | 0,024149 | DOWN (*) | ||
| PFN 1 | Profilin | 2,096222416 | 1,400267715 | 0,044221 | DOWN (*) | ||
| FSCN 1 | Fascin | – | 0,027958567 | – | UM | ||
| RDX | Radixin | 0,074884277 | – | UM | |||
| MYH 10 | Myosin-10 | 0,052986425 | – | – | UN | ||
| (iii) RhoGTP signaling | RHO A | Transforming protein rhoA precursor | – | 0,109655994 | – | UM | |
| RHO G | Rho-related GTP-binding protein RhoG precursor | – | 0,019540636 | – | UM | ||
| RAC 1 | Ras-related C3 boyulinum toxin substrate 1 | – | 0,04918568 | – | UM | ||
| ARHGDI A | Rho GDP-dissociation inhibitor 1 | 0,063887075 | 0,044089322 | 0,04036 | DOWN (*) | ||
| ARHGDI B | Rho GDP-dissociation inhibitor 2 | 0,035981062 | – | – | UN | ||
| ARHGEF 18 | Rho guanine nucleotide exchange factor 18 | 0,0087225 | – | – | UN | ||
| RHG01 | Rho GTPase-activating protein 1 | – | 0,00929341 | – | UM | ||
| ROCK 2 | Rho associate protein kinase 2 | 0,010915361 | – | – | UN | ||
Protein content was estimated as normalized emPAI values express as percentage according to Shinoda et al. The table shows proteins differentially regulated in abundance with statistical significance
*p < 0.05; **p < 0.01; ***p < 0.001, and unique proteins in normo-gravity (UN) and microgravity (UM)