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. 2018 May 10;8:7482. doi: 10.1038/s41598-018-25759-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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A representation of the two circular mitochondrial chromosomes of Ruizia karukerae. All genes are encoded in the same direction, and the 22 tRNA genes are indicated by a single-letter abbreviation. The leucine and serine tRNA genes are marked according to their anticodon sequence as L1 (trnL1-uag), L2 (trnL2-uaa), S1 (trnS1-ucu) and S2 (trnS2-uga). Primer names (see Table 2 for details) are in blue lettering, and blue curved bars indicate long PCR fragments. Brown curved bars indicate partial gene fragments obtained by 7 independent PCR reactions. Orange numbers indicate length of sequence overlap between the partial sequences and ends of long PCR fragments.