Table 2.
Primers used to sequence the complete mitochondrial genomes of Ruizia karukerae.
| Primers | Sequence (5′-3′) | Source | Size of PCR fragment | Size of overlapping region (with long PCR fragment) |
|---|---|---|---|---|
| Chromosome I | ||||
| LCO1490 | GGTCAACAAATCATAAAGATATTGG | 48 | 655 bp | 395 bp (RP7/RP8) |
| HCO2198 | TAAACTTCAGGGTGACCAAAAAATCA | 237 bp (RP1/RP2) | ||
| Nema_12S_F | GTTCCAGAATAATCGGCTA | This study | 465 bp | 122 bp (RP1/RP2) |
| Nema_12S_R | GCKATTGARGGATGYTTTGTACC | 266 bp (RP3/RP4) | ||
| Nema_16S_F_2 | TTAGTGTTGAAAAATCGTTC | This study | 678 bp | 307 bp (RP3/RP4) |
| Nema_16S_R | TCTYMCRAYGAAYTAAACTAATATC | 345 bp (RP5/RP6) | ||
| Nema_ND5_F | GTTCATAGAAGTACTTTGGTKACTGCTG | This study | 485 bp | 309 bp (RP5/RP6) |
| Nema_ND5_R | AAGACGMWAACWATAAMHAAAAGT | 348 bp (RP7/RP8) | ||
| RP1 | AGTCTGCATATGGCAGGTGTTAGC | This study | 1.5 kb | |
| RP2 | GGCTACCCGGGTACTAATCCG | |||
| RP3 | CAAACTGAAGTAAATTGGCAGGTGC | This study | 1.8 kb | |
| RP4 | CAATGGATTATGCTACTTTAATGTCC | |||
| RP5 | GGACATTAAAGTAGCATAATCCATTG | This study | 1.7 kb | |
| RP6 | GATTAAATAAGGTAACTTCCCTAAACCAC | |||
| RP7 | GATAGAGGAGGATATGAAGAAGGTAGTG | This study | 2.7 kb | |
| RP8 | GAGCTAACACCTGCCATATGCAGAC | |||
| Chromosome II | ||||
| Chroma_ND1_F_4 | GGCTTTTGTAACTCTTTATGAGCG | This study | 511 bp | 146 bp (RP13/RP14) |
| Chroma_ND1_R_2 | CCDCTNACYARYTCDCTYTC | 163 bp (RP9/RP10) | ||
| Chroma_Cob_F_2 | CARATRWSNTWTTGRGC | This study | 358 bp | 163 bp (RP9/RP10) |
| Chroma_Cob_R_2 | TAYCAYTCNGGNACAAYATG | 232 bp (RP11/RP12) | ||
| Chroma_ND4_F_1 | CATGTHGARGCDCCNAC | This study | 398 bp | 398 bp (RP11/RP12) |
| Chroma_ND4_R_3 | GTCCCAGCGTTAGTTAAAAATGTCA | 224 bp (RP13/RP14) | ||
| RP9 | GTAGAAGCCCCGACTACTGCTAG | This study | 2.8 kb | |
| RP10 | ACAAGCTTCTCCTTCCAGTCTCATG | |||
| RP11 | TGTTACATTTCTTGTTACCTTGGGC | This study | 2.3 kb | |
| RP12 | GTCCCAGCGTTAGTTAAAAATGTCA | |||
| RP13 | GTCTGTTCCAGAGGGATGGTAAAGCTCTAGC | This study | 2.8 kb | |
| RP14 | ATAACCACAAAGGCTACTGCGGGAG | |||
IUPAC nucleotide ambiguity codes used are W (A or T), R (A or G), K (G or T), S (C or G), Y (C or T), M (A or C), D (A, C or T), H (A, G or T) and N (A, C, G or T).