Fig. 5. DCs inhibit myotube differentiation.

a Immunostaining for myogenin (red), myosin heavy chain (green), and nuclei (Hoechst-blue) of myoblasts, myoblasts stimulated with LPS or co-cultured with iDC or actDCs after 24 and 96 h of incubation. The data represent three independent experiments. Bars represent 100 μm. b–d Graph representative of the fusion index, myogenin and MyoD expression presented in panel A evaluated at 24 to 96 h after incubation. e–f Fusion index of two different adult myoblast co-culture obtained from a 25 (e) and a 60-year-old (f) healthy donors evaluated at 96 h after incubation. Data shown as means ± SE of duplicates and are representative of three different experiments. *p < 0.05, **p < 0.01, and ***p < 0.001 compared to myoblasts