Table 1.
Secreted cytokine levels in the supernatants from co-cultures of myoblasts and myotubes with DCs during proliferation and differentiation
| A | GM-CSF (pg/mL) | IFNa2 (pg/mL) | IL-10 (pg/mL) | IL-13 (pg/mL) | IL-6 (pg/mL) | IL-8 (pg/mL) | CCL2 (pg/mL) | TNF-a (pg/mL) |
|---|---|---|---|---|---|---|---|---|
| Myoblasts | 33.5 ± 2.1 | 13 ± 0.1 | 3.2 ± 0.1 | 3.2 ± 0.1 | 16079 ± 997.2 | 677 ± 36..8 | 9185 ± 559.7 | 11.6 ± 9.7 |
| Myoblasts + LPS | 198.5 ± 20.5 | 6.5 ± 0.7 | 3.2 ± 0.1 | 3.2 ± 0.1 | 15312.5 ± 888.8 | 5465.5 ± 522.6 | 10211 ± 1478.1 | 47.3 ± 25.5 |
| Myoblasts + iDC | 117.5 ± 14.9 | 16 ± 4.2 | 69 ± 8.5 | 8.6 ± 7.6 | 15811 ± 97.6 | 9093 ± 207.9 | 10872 ± 1020.1 | 146.5 ± 41.1 |
| Myoblasts + actDCs | 273 ± 28.3 | 9 ± 5.7 | 148.50 ± 6.4 | 34.5 ± 4.9 | 16134 ± 521.9 | 13960 ± 1233.2 | 10855.5 ± 565.9 | 44.8 ± 8 |
| iDC | 128.5 ± 20.5 | 3.1 ± 0.1 | 41 ± 4.2 | 100.5 ± 0.7 | 6.95 ± 0.5 | 9218.5 ± 1266.4 | 10744.3 ± 345.6 | 172.5 ± 75.77 |
| actDCs | 198 ± 22.6 | 7 ± 1.4 | 176 ± 14.1 | 93 ± 26.9 | 38.5 ± 13.3 | 13156 ± 2023.7 | 11283.5 ± 607.9 | 34.8 ± 4.1 |
| B | GM-CSF (pg/mL) | IFNa2 (pg/mL) | IL-10 (pg/mL) | IL-13 (pg/mL) | IL-6 (pg/mL) | IL-8 (pg/mL) | CCL2 (pg/mL) | TNF-a (pg/mL) |
|---|---|---|---|---|---|---|---|---|
| Myotubes | 17 ± 9.2 | 22.7 ± 3.2 | 3.2 ± 0.1 | 3.2 ± 0.1 | 4129.3 ± 69.5 | 1733.3 ± 355.6 | 9348 ± 383.3 | 3.2 ± 0.1 |
| Myotubes + LPS | 86.7 ± 23.6 | 24 ± 1.7 | 3..2 ± 0.1 | 3.2 ± 0.1 | 4922.3 ± 489.9 | 10940.7 ± 2241.6 | 9060.7 ± 1043.6 | 10.7 ± 4.2 |
| Myotubes + iDC | 148 ± 21.4 | 18.3 ± 2.1 | 127.3 ± 14.7 | 25.7 ± 2.9 | 879.7 ± 146.7 | 11003.3 ± 1276.9 | 8716 ± 1053 | 59 ± 11.4 |
| Myotubes + actDCs | 198.7 ± 30.1 | 16.7 ± 3.2 | 426 ± 17.4 | 19.7 ± 10.8 | 1447.3 ± 847.7 | 14099 ± 260.8 | 9513.3 ± 620.7 | 59.7 ± 8 |
| iDC | 3.2 ± 0.1 | 9 ± 4.6 | 98.7 ± 4.9 | 6.3 ± 2.1 | 190 ± 6.2 | 14914 ± 1358.7 | 7407.7 ± 409.9 | 61.3 ± 9.8 |
| actDCs | 32.7 ± 6.7 | 13.3 ± 1.5 | 682.3 ± 36.1 | 14.7 ± 5.5 | 870.3 ± 186.7 | 17411.7 ± 887.8 | 10517 ± 232.5 | 137.7 ± 14.2 |
Supernatants obtained from myoblasts, myoblasts stimulated with LPS, or co-cultured with iDC or actDCs, and supernatants obtained from iDC or actDCs single culture were analyzed by Luminex for GM-CSF, IFN-2α, IL-10, IL-13, IL-6, IL-8, CCL2, and TNF-α. The results in the tables are expressed as absolute numbers in pg/mL of supernatant, in proliferation (A) and in differentiation (B). Data are expressed as means ± SE and are representative of four different experiments.