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. 2018 May 10;8:7459. doi: 10.1038/s41598-018-25798-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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eIF4G1 is required for cell growth, colony formation, expression of EMT genes and cell migration in PCa Cells: Loss of Function studies following knockdown of eIF4G1 using siRNA (a,b). Cell viability was measured by crystal violet assay at 48–96 h with siControl and sieIF4G1, the graph showed absorbance at 560 nm for LNCaP (a) and C4-2B (b). (c) Cell proliferation assay for LNCaP and C4-2B (d) done by MTT assay at 24–96 h with siControl and sieIF4G1. (e) Colony formation for LNCaP and C4-2B (f) siControl and sieIF4G1, Lower panels: Colonies were counted using an ImageJ software. Error bar represents ± SD. (g) Representative image of immunoblotting for LNCaP and C4-2B for N-Cadherin & Snail 1 with siControl and sieIF4G1. (h) Representative image and graph of Transwell cell migration assays on LNCaP (h) & C4-2B (i) with Vector Control (VC) & sheIF4G1. p-values are indicated as *<0.05, **<0.01, ***<0.001.