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. 2018 May 10;9(5):537. doi: 10.1038/s41419-018-0565-2

Fig. 4. miR-455-3p inhibits cartilage degenerate process in vitro and in vivo.

Fig. 4

hBMSCs were transfected with miR-455-3p agomir induced to differentiate to chondrocytes in micromass culture. Safranin O was strained for 3, 7, 14, 21, 28, and 35 days of chondrogenic differentiation (a). We extracted the RNA on days 28 of the chondrogenic differentiation to detect the expression of miR-455-3p (b), COL2A1, COL11A1, and SOX6 (c) by RT-PCR. U6 and GAPDH expression levels were measured and used as internal controls for microRNA and mRNA expression, respectively. Wild-type (WT) mice (d) and miR-455-3p deletion (KO) mice (e) were stained with Safranin O at 6 months of age, scale bar, 100 μm. Average thicknesses of total articular cartilage in femoral head were quantified. miR-455-3p deletion mice showed more hypertrophic chondrocytes (black arrow) and thinner cartilage than WT mice (f). Data are presented as means ± SD of the results obtained from triplicate samples. *P < 0.05; **P < 0.001 vs negative controls, respectively