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. 2018 May 10;9(5):544. doi: 10.1038/s41419-018-0601-2

Fig. 2. CC2C6 inhibits anti-phagocytic signal of CD47.

Fig. 2

a Cell Tracker-labelled WT or CD47-/-Jurkat cells were untreated or treated with B6H12 or CC2C6 prior to their incubation with primary mouse macrophages (F4/80 labeled) for 2 h. In two samples, macrophages were treated with the SIRPα-neutralizing antibody, P84, prior and during incubation with WT or CD47−/− cells. Shown is the flow cytometry plots of the mixed population, where double positive cells indicate Jurkat cells phagocytosed by macrophages. b The phagocytic index for the data shown in (a) was calculated as follows: 100 × (CellTracker and F4/80 double + ve cells) / total macrophages. Error bars represent the standard deviation for n = 3 replicates; *p < 0.01 relative to untreated WT. Representative of two independent experiments. c Hemagglutination assay of human erythrocytes incubated with increasing concentrations of the CD47-antibodies, CC2C6, B6H12 or BRIC-126. d DIC and immunofluorescence imaging of WT or CD47−/− cells treated with CC2C6 or B6H12 and mounted in suspension. Green: CD47; Blue: DAPI. Bar: 10 μM