Fig. 6. Modulation of LIN7A expression abolished miR-501-3p-mediated cellular activities in vitro.

a Western blot showed the expression of LIN7A protein in HCCLM3-miR-501-3p cells that were transfected with LIN7A vector or PLC/PRF/5-anti-miR-501-3p cells that were transfected with shLIN7A vector and their corresponding controls. **P < 0.01. b Proliferation of HCCLM3-miR-501-3p that were transfected with LIN7A and PLC/PRF/5-anti-miR-501-3p cells that were transfected with shLIN7A vector and their corresponding controls was determined by CCK-8 assay. **P < 0.01. c Wound-healing assay and d transwell assay were performed to determine the effects of LIN7A on the migration and invasion of HCC cells. Scale bar: 100 μm, **P < 0.01. e LIN7A restoration decreased the expression of E-cadherin and increased the levels of N-cadherin, Vimentin, and Snail in miR-501-3p-overexpressing HCCLM3 cells. LIN7A knockdown abolished the effects of miR-501-3p loss on EMT process of PLC/PRF/5 cells. **P < 0.01. Data depicts the mean ± standard deviation and are representative of three independent experiments