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. 2018 May 10;4:54. doi: 10.1038/s41420-018-0061-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a The mitochondria-specific dye dihydrorhod-2 AM was used for the flow cytometric calcium measurements. The dark grey graphs represent the untreated controls, the white graphs the glutamate-treated samples, and the light grey areas the overlay of both graphs. b Here, the right shift in Rhod-2 fluorescence in response to 11 mM glutamate was quantified. The bar graph shows one representative experiment with three replicates per sample (mean + SD; 10,000 cells per replicate). ***p < 0.001 compared to glutamate-treated ctrl (ANOVA, Scheffé’s-test)