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. 2018 May 10;4:54. doi: 10.1038/s41420-018-0061-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Representative phase-contrast images of glutamate-treated HT22 cells illustrate changes in cell morphology. Scale bar 25 µm. b A representative real time cell impedance measurement over 30 h after the treatment with 8 mM glutamate is shown. Eight replicate wells per condition are displayed as mean ± SD. c Before cell viability was determined by MTT assay, HT22 cells were challenged with 8 mM glutamate for 16 h. Values from 8 wells per condition are presented as mean + SD. The absorbance under control conditions is set as 100%. d The bar graph shows the percentage of annexin V and AV/PI positive cells. One representative experiment with three replicates per sample is depicted (mean + SD; 10,000 cells per replicate). e In addition, the dot plots exemplify the subdivision into AV (lower right quarter) and AV/PI positive cells (upper right quarter). AV annexin V, PI propidium iodide. ***p < 0.001 compared to glutamate-treated ctrl (ANOVA, Scheffé’s-test)