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Examples of positive and negative cells. CEP8: orange; CD45: red; DAPI: blue; positive cells: number of FISH signal dots ≥3 (i.e., hyperdiploid), CD45 negative, and DAPI positive. (A) Positive cell (CTC); (B) negative cell (leukocyte); (C) negative cell (leukocyte). FISH, fluorescence in situ hybridization; CEP8, chromosome enumeration probe 8; CTC, circulating tumor cell.
