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. 1999 Nov;121(3):929–938. doi: 10.1104/pp.121.3.929

Figure 5.

Figure 5

AtVAM3p localizes to the PVC in Arabidopsis root cells. Cryosections of the root tip of transgenic Arabidopsis seedlings expressing either T7-AtPEP12 or T7-AtVAM3 were prepared and stained with T7-monoclonal or specific antisera to AtPEP12p (C-terminal specific) or AtVAM3p. In all cases, AtVAM3p is represented by 10-nm gold particles (arrows), while AtPEP12p is represented by 5-nm gold particles (arrowheads). A, Cryosections of T7-AtVAM3-expressing transgenic plants stained with T7-monoclonal antibodies detected with 10-nm gold. B, Cryosections of T7-AtVAM3-expressing transgenic plants stained with no antisera followed by 10-nm gold. C, Cryosections of T7-AtVAM3-expressing transgenic plants stained with T7-monoclonal antibodies detected with 10-nm gold and with affinity-purified AtPEP12p antiserum (C-terminal specific) detected with 5-nm gold. D and E, Cryosections of T7-AtPEP12-expressing transgenic plants stained with T7-monoclonal antibodies detected with 5-nm gold and with affinity-purified AtVAM3p antiserum detected with 10-nm gold. F, Cryosections of T7-AtPEP12-expressing transgenic plants stained with no antibodies followed by 10-nm gold and preimmune serum for AtPEP12p detected with 5-nm gold. Bar = 500 nm.