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. 2018 May 10;8:7476. doi: 10.1038/s41598-018-25895-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Linear correlation of cell number and dCq value of Control DNA. After coWGA of various cell numbers, dCq is determined in real-time PCR. The dCq value of Control-DNA is plotted against the common logarithm (log₁₀) of cell number. The standard deviation is given as error bars. The dCq value of co-amplified Control-DNA clearly correlates with the common logarithm of cell number used in WGA. (b) Frequency of WGA reactions characterized by dCq intervals of co-amplified Control-DNA: WGA was performed using Control-DNA and a cell dilution of 0.2 cells/WGA. As expected from Poisson distribution, most reactions did not contain any cell and resulted in a low dCq value. dCq values of ~7 represents WGA reaction containing at least a single cell. (c) Comparison of experimental data and Poisson distribution after WGA with 0.2 cells/WGA in average. WGA reaction with dCq value < 5.5 correlated with expected fraction of WGA reactions containing 0 cells. The second cluster of WGA reactions (dCq > 5.5) correlated with expected fraction of WGA reactions containing at least one cell. (d) Distribution of dCq value intervals of 0.2 cycles of WGA reactions containing cells.