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. 2018 May 10;8:7476. doi: 10.1038/s41598-018-25895-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fraction of aligned reads plotted over dCq value of Control-DNA after WGA. About 2.3 × 10⁶ reads are aligned per library to the human genome. The fraction of reads that are aligned to the human genome changes with the degree of damaging of cells. The dCq value of ControlDNA spiked during WGA of single human cells correlates exponentially with the fraction of the reads aligned to the human genome.